University of Cambridge > Talks.cam > Babraham Seminar > "Vectorbuilder: Revolutionising Vector Design & Custom Cloning" (25 min seminar) followed by "Advanced Technologies For Rapid Generation Of Custom Designed Animal Models" (25 min seminar)

"Vectorbuilder: Revolutionising Vector Design & Custom Cloning" (25 min seminar) followed by "Advanced Technologies For Rapid Generation Of Custom Designed Animal Models" (25 min seminar)

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2×25 minute seminars

VectorBuilder: Revolutionising Vector Design and Custom Cloning (25 min Seminar) - The only reason cloning is not outsourced is because of cost and a need for communicating the precise details of each individual vector design for the experimental purpose. With VectorBuilder, this is no longer a problem. I will introduce and perform a live demo of VectorBuilder (www.vectorbuilder.com). VectorBuilder is a revolutionary new online tool that not only allows you to create your custom vectors with ease but also allows you to simply order them within minutes at competitive prices and rapid turnaround times. With over 110,000 vectors already delivered, Vectorbuilder offers:

• Plasmids • Lentivirus • Adenovirus • AAV (adeno associated virus) • Tet-on/off • CRISPR • Cre-lox • Massive collections of ORFs • Tissue-specific promoters • Add tags/reporters/drug-selectable markers • Virus packaging – Lenti, Adeno, AAV , MMLV • BAC modification • Library construction

Advanced Technologies For Rapid Generation Of Custom-Designed Animal Models For Your Research (25 min Seminar) – While the generation of transgenic mouse and rat models clearly provides invaluable insights into human disease, the process of generating transgenic animals is often complicated, time-consuming and costly. Cyagen (www.cyagen.com) is the world’s leading provider of custom-designed transgenic mice and rats offering a one-stop solution to all your animal needs. To date, we have delivered over 18,500 animal models.

In this seminar, I will provide an overview of the current State-of-the-art as well as draw on own academic experience to give an overview of our technologies including: · PiggyBac transgenic mice and rats – single copy transgene integration · TurboKnockout® – ES cell targeted cKO/KI mice in 6-8 months · CRISPR -mediated genome editing – Knockouts/knockins (Up to 8Kb)

This talk is part of the Babraham Seminar series.

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