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LMB Seminar Series - CryoEM of proton pumping macromolecular machines

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Electrochemical proton gradients across energized membranes play central roles in numerous cellular processes. These gradients are established by large membrane-embedded protein complexes.V-type ATPasesare responsible for acidification of intracellular compartments including endosomes, lysosomes, the Golgi, and exocytic vesicles. Electron transport chain complexes energize the inner membranes of mitochondria and the plasma membranes of bacteria to drive production of cellular ATP during oxidative phosphorylation. Many of these protein complexes are highly dynamic, complicating their structural analysis. We have isolated a number of these assemblies from bacterial, yeast, and mammalian sources, and examined them by electron cryomicroscopy (cryoEM) to understand their function and regulation. In order to facilitate this process, we also develop new biochemical, specimen preparation, and computational approaches for cryoEM.

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