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Immune profiling the human NK cell repertoire using mass cytometry (CyTOF)

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Host: Ashley Moffett (am485@cam.ac.uk)

The Parham laboratory investigates the biology, genetics and evolution of MHC class I molecules and Natural Killer (NK) cell receptors (NKRs).

To provide a framework for understanding how perturbations in expression of NKRs influence disease pathogenesis, we defined the phenotypic heterogeneity of NK cells in 22 healthy individuals, including 5 sets of monozygotic twins.

Using mass cytometry, surface expression of 38 markers specific for NKRs and lineage markers to identify B cells, T cells and myeloid cells were examined. KIR and HLA class I genotypes were determined by Luminex, and KIR gene content was further assessed using pyrosequencing, in order to better appreciate how these cells are educated and subsequently regulated.

This talk is part of the Immunology in Pathology series.

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