University of Cambridge > > BSS Formal Seminars > Enhancing single-molecule detection with DNA origami nanostructures

Enhancing single-molecule detection with DNA origami nanostructures

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If you have a question about this talk, please contact Dr Eileen Nugent.

In recent years, we have combined DNA nanotechnology with single-molecule fluorescence to create functional single-molecule devices such as nanoscopic rulers for superresolution microscopy and energy transfer switches[1-4]. DNA origamis are also used to improve single-molecule detection by fluorescence enhancement with nanoantennas or by single-molecule placement in zeromode waveguides using nanoadapters[5, 6]. Especially, fluorescence enhancement with self-assembled nanoparticles holds great potential for single-molecule detection at higher concentrations but also for diagnostic applications [7]. We will discuss recent advancement in fluorescence enhancement and how to disentangle the complex factors that influence the fluorescence of single molecules near metallic nanostructures[8, 9].


1. J. J. Schmied, M. Raab, C. Forthmann, E. Pibiri, B. Wunsch, T. Dammeyer and P. Tinnefeld, Nat Protoc, 2014, 9, 1367-1391.

2. J. J. Schmied, C. Forthmann, E. Pibiri, B. Lalkens, P. Nickels, T. Liedl and P. Tinnefeld, Nano Lett, 2013, 13, 781-785.

3. J. J. Schmied, A. Gietl, P. Holzmeister, C. Forthmann, C. Steinhauer, T. Dammeyer and P. Tinnefeld, Nat Methods, 2012, 9, 1133-1134.

4. I. H. Stein, C. Steinhauer and P. Tinnefeld, J Am Chem Soc, 2011, 133, 4193-4195.

5. E. Pibiri, P. Holzmeister, B. Lalkens, G. P. Acuna and P. Tinnefeld, Nano Lett, 2014, 14, 3499-3503.

6. G. P. Acuna, F. M. Moller, P. Holzmeister, S. Beater, B. Lalkens and P. Tinnefeld, Science, 2012, 338, 506-510.

7. P. Holzmeister, G. P. Acuna, D. Grohmann and P. Tinnefeld, Chem Soc Rev, 2014, 43, 1014-1028.

8. J. V. Pellegrotti, G. P. Acuna, A. Puchkova, P. Holzmeister, A. Gietl, B. Lalkens, F. D. Stefani and P. Tinnefeld, Nano Lett, 2014, 14, 2831-2836.

9. P. Holzmeister, E. Pibiri, J. J. Schmied, T. Sen, P. Acuna Guillermo and P. Tinnefeld, Nat Commun, 2014, accepted.

This talk is part of the BSS Formal Seminars series.

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