University of Cambridge > Talks.cam > Biological and Statistical Physics discussion group (BSDG) > Determining and approaching the physical limits of electron cryomicroscopy in biology

Determining and approaching the physical limits of electron cryomicroscopy in biology

Add to your list(s) Download to your calendar using vCal

If you have a question about this talk, please contact William Grant.

In spite of recent advances in electron cryomicroscopy (cryo-EM), the structures of many proteins cannot be determined by cryo-EM because the individual protein molecules move during electron irradiation. This blurs the images so they cannot be aligned with each other to improve the signal to noise ratio and calculate a 3D density map. I will discuss the types of movement at various length scales that occur in biological specimen during high energy electron irradiation, and show how reducing this movement leads to improved micrographs and density maps. Further, I will discuss several physical limits important to cryo-EM and how they determine the types of specimen which will ultimately be amenable to atomic resolution imaging with electrons.

This talk is part of the Biological and Statistical Physics discussion group (BSDG) series.

Tell a friend about this talk:

This talk is included in these lists:

Note that ex-directory lists are not shown.

 

© 2006-2017 Talks.cam, University of Cambridge. Contact Us | Help and Documentation | Privacy and Publicity