University of Cambridge > Talks.cam > Plant Sciences Departmental Seminars > Using the auxin-inducible degron in yeast to understand the spliceosome cycle

Using the auxin-inducible degron in yeast to understand the spliceosome cycle

Add to your list(s) Download to your calendar using vCal

If you have a question about this talk, please contact ps753.

Pre-mRNA splicing is essential for eukaryotic cells and is catalyzed by a very large RNA /protein particle called the spliceosome. During my PhD, my initial research aim was to identify proteins potentially involved in linking splicing with transcription. To this end, I used the auxin-inducible degron technique to quickly deplete proteins of interest, and chromatin immunoprecipitation to measure in vivo assembly of the spliceosome. I discovered that knocking-down splicing proteins can quickly lead to strong secondary effects that can mask the primary consequence of the depletion. I will explain where these secondary effects are coming from, suggest one way to avoid them and discuss the implications of this finding. Towards the end of my talk, I will present the plans I have of applying pre-mRNA splicing techniques to engineer the thiamine-responsive riboswitch of Chlamydomonas reinhardtii. My goal is to create novel molecular tools to control transgene expression in plants.

This talk is part of the Plant Sciences Departmental Seminars series.

Tell a friend about this talk:

This talk is included in these lists:

Note that ex-directory lists are not shown.

 

© 2006-2018 Talks.cam, University of Cambridge. Contact Us | Help and Documentation | Privacy and Publicity