Developing ways to image single proteins in living cells

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• Srinjan Basu (Dept of Biochemistry) and Aleks Ponjavic (Dept of Chemistry)
• Wednesday 04 October 2017, 18:30-20:00
• Postdoc Centre, 16 Mill Lane.

If you have a question about this talk, please contact Leonor Miller-Fleming.

In 2014, the Nobel Prize in Chemistry was awarded for developments in the field of light microscopy that have allowed the detection of single fluorophores at resolutions of 20 nm, well below the traditional diffraction limit of 250 nm.

Here, we describe how to use one of these approaches called single-molecule localization microscopy (SMLM) to achieve such high resolutions and how recent developments have allowed us study the live-cell dynamics of single fluorophore-tagged proteins initially in bacteria and more recently in mammalian cells. Finally, we discuss how the imaging of single proteins interacting with DNA or moving on the cell membrane can help us understand how cells respond to their environment?  

This talk is part of the "Life Sciences Masterclass" series.

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