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Methods for rapid 3D fluorescence microscopy

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In fluorescence microscopy, 3D image data is typically formed by acquiring a series of 2D images while changing the focal plane. This process is slow as it involves mechanically moving the sample or the objective, and as such forms a bottle neck for volumetric imaging. Here I present two applications of remote focusing that can significantly speed up volumetric imaging, both in two photon raster scanning microscopy and in light-sheet fluorescence microscopy.

I will further present a novel way to form projections of microscopic 3D objects under varying viewing angles. This method allows capturing views of a 3D sample at ~hundredfold increased speed compared to conventional 3D image acquisition. This can be valuable if the object is sufficiently sparse, and one does not need the full 3D information. But I will also show that it is possible to obtain three-dimensional reconstructions from a few rapidly acquired projections.

All three methods will be carefully characterized for their optical performance and examples of rapid biomedical imaging will be given.

This talk is part of the Physical Chemistry Research Interest Group series.

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