Abstract

consequence of the non-motile nature of plant cell is that the positioning of new cell walls during cytokinesis will influences cell geometry, tissue topology, and patterning. Reciprocally, cell geometry, local or global mechanical stress, or organ growth properties can influence the positioning of new cell walls. Because these parameters are highly interconnected, identifying the causative factor regulating the positioning of new cell walls is often difficult. To dissect these interconnected relationships between division orientation, cell geometry, growth and mechanical stress, we used Arabidopsis thaliana transgenic material to induce the formation of ectopic divisions at various developmental stages of cotyledons with distinct growth properties, cell geometry, and mechanical stress pattern. We used time-lapse imaging and mechanical perturbation to further investigates the implication of mechanical stress in cell division orientation. Finally, we used computational modelling to predict the orientation of tensile stress in real cell templates. Altogether our approaches enabled us to examine the relationship between these parameters and cell division orientation.