Phylogenomic analysis of tobacco; identifying the genes selected during domestication

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• Kieron Edwards, Applied Molecular Breeding manager of Advance Technologies
• Friday 22 October 2010, 13:00-13:30
• Department of Plant Sciences, Large Lecture Theatre.

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Nicotiana tabacum (2n = 4x = 48) is an allotetraploid derived from a hybridisation of the ancestral species N. sylvestris and N. tomentosiformis around 0.2 million years ago. Polyploidisation is very common in the plant kingdom and is likely to have made a significant contribution to speciation. There are several possible fates for genes duplicated during polyploidisation including retaining their original function, being lost or silenced, or undergoing functional or regulatory diversification. To gain an insight into the fate of genes duplicated in tobacco, the leaf transcriptome of N. tabacum and its ancestral parents was assayed using next generation sequencing. A phylogenetic analysis pipeline was developed to analyse the relationship of the tabacum genome to its two putative parental genomes in a set of 11000 contigs where there was at least one representative sequence from each species. The implication of these results will be discussed.

This talk is part of the Plant Sciences Research Seminars series.

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