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Recombinatorial cloning tools for probing Staphylococcus aureus

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Many bacterial pathogens are problematic to analyse due to a paucity of molecular tools and intrinsic properties of the organisms such as poor transformation efficiencies or low recombination frequencies. We have engineered vectors compatible with recombinatorial cloning for plasmid and chromosomally integrated expression and validated them in a number of organisms using reporter gene constructs. A mutagenesis system combining recombinatorial cloning with marker excision, mediated by ΦC31 integrase, to make sequential knockout mutants in Gram positive pathogens has facilitated the study of the intracellular lifestyle of Staphylococcus aureus.

This talk is part of the Departmental Seminar Programme, Department of Veterinary Medicine series.

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