Abstract

Abstract: Electrochemistry has been widely applied into the field of biosensor in the past two decades. Numerous novel sensors based on measuring electrochemical current, capacitance and impedance have been successfully developed. They have been considered promising as next generation of biosensors due to their simplicity, low cost and capability of integration, over conventional technologies. In this work the homogeneous enzymatic system has been systematically studied in the framework of cyclic voltammetry as a basis to develop amperometric biosensors based on the use of enzyme as transducer. A reversible electrode reaction with fast enzyme process is modeled and the differential equations are solved numerically using finite element software. Methods have been developed to extract kinetic parameters such as mediator reaction rate constant, Michaelis-Menten constant and so on. The glucose / glucose oxidase / ferrocene monocarboxylic acid system is applied as an example system to validate the results. Good agreement has been achieved over a wide range of experimental conditions and hence demonstrated that the method can be used to study general enzyme/mediator/substrate type of biosensor.