Abstract

Nanopores are on the brink of fundamentally changing DNA sequencing. At the same time, DNA origami provides unprecedented freedom in molecular design. Here, I suggest why a combination of solid-state nanopores and DNA nanotechnology will lead to exciting new experiments. I will introduce a novel way to detect and identify single proteins by using DNA carriers. With DNA nanotechnology we buitd carriers that allow for digitally encoding information and thus unambiguous protein identification and concentration measurements down to nanomolar levels. This opens the pathway to massively multiplexed nanopore sensing of proteins [1] and other small biomolecules.

[1] N. A. W. Bell and U. F. Keyser. Digitally encoded DNA nanostructures for multiplexed, single-molecule protein sensing with nanopores. Nature Nanotechnology, published online, 2016.