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Approaches to imaging fast biological events

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Speaker 1: Dr Michael Shaw (NPL Senior Research Scientist/ UCL Senior Research Associate)

Title: High speed 3D imaging using light field microscopy for neurobiological and behavioural studies of C. elegans

Speaker 2: Dr Vikas Trivedi (Herchel Smith Postdoctoral Fellow, Department of Genetics, University of Cambridge)

Title: 2-photon light sheet imaging of zebrafish heart

Abstract: The model organism C. elegans is used in a range of fundamental studies of physiology and development as well as drug screening. However, to fully capture the behaviour and neurological response of the organism requires high speed volumetric imaging which is impossible using most conventional microscopy techniques. In this talk I will describe how we have used light field microscopy, in which multiple perspective views of the organism are captured simultaneously using a microlens array placed at the native image plane of a conventional microscope, to measure neuronal activity and quantify the locomotion of C. elegans in 3D. I will discuss some of the strengths and limitations of the technique and discuss how it is being used at UCL to investigate the process of mechanosensation.

This talk is part of the Cambridge Advanced Imaging Seminars series.

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