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Professor Stephen J. Fey, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark
Tuesday 07 February 2017, 08:00-08:30
Metabolic Reprogramming in Microgravity 3D Spheroids
- đ¤ Speaker: Professor Stephen J. Fey, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark
- đ Date & Time: Tuesday 07 February 2017, 08:00 - 08:30
- đ Venue: Online
Abstract
Microgravity spheroids reach a metabolic equilibrium after 18 days in culture and maintain this equilibrium for at least 24 days thereafter. Spheroids grown from HepG2/C3A cell line are large (ca. 0.8 – 1mm in diameter), reproducible, and show significant structural and ultrstructural similarities to human liver and differences compared to the same cells grown in the same conditions in classical tissue culture conditions. Most importantly, they are highly tissue mimetic. They are more accurate than primary human hepatocytes for predictive toxicology. They recover epigenetic markings and histone clipping seen in vivo and they display clear metabolic reprogramming.
Series This talk is part of the Three-dimensional cell culture: Innovations in tissue scaffolds and biomimetic systems series.
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