Abstract

The use of electrospun nanofibres for guided bone regeneration or bone scaffolds align with next-generation healthcare, especially as electrospun nanofibres are highlighted as being particularly effective in tissue engineering. However, optimization of the electrospinning process for cell growth and their interaction with nanofibre surfaces is yet to be determined. In our studies, we show the interaction between osteoblasts and osteoblast-derived mineralized nodule formation on the nanofibre membrane and visualized it using 3D tomography based on focused ion beam and scanning electron microscopies (FIB-SEM). This new approach in high resolution visualization of cells growth on electrospun nanofibers, and potentially other biomaterials allows designing new biomaterials for a range of clinically important applications.