Determining and approaching the physical limits of electron cryomicroscopy in biology

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• Dr. Christopher J. Russo ( MRC Laboratory for Molecular Biology, U.K.)
• Thursday 02 March 2017, 12:00-13:00
• TCM Seminar room, 530 Mott building.

If you have a question about this talk, please contact William Grant.

In spite of recent advances in electron cryomicroscopy (cryo-EM), the structures of many proteins cannot be determined by cryo-EM because the individual protein molecules move during electron irradiation. This blurs the images so they cannot be aligned with each other to improve the signal to noise ratio and calculate a 3D density map. I will discuss the types of movement at various length scales that occur in biological specimen during high energy electron irradiation, and show how reducing this movement leads to improved micrographs and density maps. Further, I will discuss several physical limits important to cryo-EM and how they determine the types of specimen which will ultimately be amenable to atomic resolution imaging with electrons.

This talk is part of the Biological and Statistical Physics discussion group (BSDG) series.

This talk is included in these lists:

• Biological and Statistical Physics discussion group (BSDG)
• Combined TCM Seminars and TCM blackboard seminar listing
• PMRFPS's
• TCM Seminar room, 530 Mott building

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