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SUMMARY:Reconstitution and understanding of mammalian oogenesis - Professo
 r Katsuhiko Hayashi\, Department of Stem Cell Biology and Medicine\,  Grad
 uate School of Medical Sciences\,  Kyushu University\, Japan
DTSTART:20190319T110000Z
DTEND:20190319T120000Z
UID:TALK121162@talks.cam.ac.uk
CONTACT:Dr Walfred Tang
DESCRIPTION:The female germ line undergoes a unique sequence of differenti
 ation processes that finally endows the egg with totipotency. The reconsti
 tution in vitro of oogenesis using pluripotent stem cells\, which eventual
 ly produces functional oocytes\, has long been sought in reproductive biol
 ogy and developmental biology\, since it would contribute to not only a be
 tter understanding of mechanisms underlying totipotency\, but also an alte
 rnative source of gametes for reproduction. \nWe developed a culture syste
 m that reconstituted the entire process of oogenesis from mouse pluripoten
 t stem cells\, yielding in vitro-generated eggs that were capable of full-
 term development. In the culture system\, primordial germ cells were induc
 ed from ESCs/iPSCs\, and then were aggregated with somatic cells of fetal 
 ovaries. The aggregates\, named reconstituted ovaries\, passed through sev
 eral culture stages\, which in total took approximately 5 weeks. After the
 se stages\, a number of mature oocytes were produced in the reconstituted 
 ovaries. \nThe culture system is extremely useful\, as genetic manipulatio
 n can be done in pluripotent stem cells and outcome can be seen in culture
 . In the seminar\, I will introduce recent advances in egg production from
  pluripotent stem cells and update current results to address mechanisms u
 nderlying oocyte differentiation. \n
LOCATION:Biochemistry Lecture Theatre\, Sanger Building\, Tennis Court Roa
 d
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