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SUMMARY:Developing a method for meiotic cell -specific gene expression and
  chromatin profiling in Arabidopsis thaliana. - Artemis Perraki\, Andreuzz
 a group
DTSTART:20200227T130000Z
DTEND:20200227T133000Z
UID:TALK131725@talks.cam.ac.uk
CONTACT:71002
DESCRIPTION:Meiosis is essential for sexual reproduction\, as it produces 
 haploid cells that will differentiate into gametes\, by a single round of 
 replication and two rounds of chromosome segregation. During meiosis homol
 ogous chromosomes pair and recombine\, which can lead to reciprocal exchan
 ge of genetic material fragments or crossovers (COs)\, a process that gene
 rates genetic variation between generations. Therefore\, identifying the m
 olecular controls of meiosis is of great interest for agriculture and crop
  breeding (Tock and Henderson\, 2018\, Mercier et al.\, 2015).\n  \nIn flo
 wering plants\, meiotic cells develop within layers of somatic tissue (sub
 -epidermal cells) in the ovules and anthers. However\, purifying these sma
 ll population of cells represents a major limitation in the characterizati
 on of plant meiosis. The aim of my project is to separate meiotic cells fr
 om somatic cells of floral organs in order to perform meiotic cell specifi
 c gene expression analysis and chromatin profiles. For this\, I am current
 ly optimising for meiosis\, a specific purification approach called INTACT
  (isolation of nuclei tagged in specific cell types)\, that allows the aff
 inity-based isolation of nuclei from individual cell types of a tissue (De
 al and Henikoff\, 2010). \n\n# Deal RB and Henikoff S. A simple method for
  gene expression and chromatin profiling of individual cell types within a
  tissue. Dev Cell. 2010 Jun 15\;18(6):1030-40.\n# Mercier R\, Mézard C\, 
 Jenczewski E\, Macaisne N and Grelon M. The molecular biology of meiosis i
 n plants. Annu Rev Plant Biol. 2015\;66:297-327.\n# Tock and Henderson. Ho
 tspots for initiation of meiotic recombination. Front Genet. 2018\; 9: 521
 .\n
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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