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SUMMARY:Refining super-resolution microscopy: lessons learnt from imaging 
 intracellular signalling nanodomains - Dr. Izzy Jayasinghe\, UKRI Future L
 eader Fellow\, University of Leeds
DTSTART:20191112T130000Z
DTEND:20191112T140000Z
UID:TALK133714@talks.cam.ac.uk
CONTACT:Cavendish Inspiring Women
DESCRIPTION:This talk will outline a decade of research in Cell Biology an
 d Biophysics which has utilised super-resolution microscopy – a family o
 f optical imaging techniques which strive to resolve structures which were
  previously limited to electron microscopy exclusively. Intracellular sign
 alling ‘nanodomains’ are some of the most commonly imaged structures o
 ver the last few decades. In muscle cells which make up the tissue of the 
 heart\, these nanodomains orchestrate a series of calcium signals which un
 derpin the heartbeat. \n\nIn this seminar\, I will outline how the adaptat
 ion of chemical photo-switching of aromatic fluorescent labels\, together 
 with the first generation of super-resolution microscopy modalities (e.g. 
 dSTORM) enabled us to acquire the first optically-resolved images of nanod
 omains\, a decade ago. \n\nOver the last few years\, there has been a wave
  of newer super-resolution microscopy modalities which have transformed th
 e way we visualise nanodomains. By refining and re-developing these method
 s\, we have been able to map\, count and distinguish individual ion channe
 ls and regulatory proteins\, which make up the nanodomains in the heart. W
 e can also visually identify distinct chemical signatures of individual io
 n channels in situ\, perform spatial statistics on their interactions duri
 ng the genesis of calcium signals and simulate these calcium signals in si
 lico\, at an unprecedented level of spatial and temporal detail. I will de
 tail the adaptations and refinements of DNA-PAINT and “Enhanced Expansio
 n Microscopy” which have enabled these observations. \n\nFinally\, I wil
 l also introduce ‘sandSTORM’ a brand new method which has been develop
 ed in my team\, exploiting the spontaneous photoluminescence properties of
  nanodiamonds\, as one of the fastest super-resolution microscopy modaliti
 es.
LOCATION: Small Lecture Theatre\, Cavendish Laboratory\, J.J. Thomson Aven
 ue
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