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SUMMARY:Mechanisms of centriole assembly - Pierre Gönczy\, Swiss Federal 
 Institute of Technology Lausanne (EPFL)\, Switzerland
DTSTART:20210622T100000Z
DTEND:20210622T110000Z
UID:TALK160732@talks.cam.ac.uk
CONTACT:Scientific Meetings Co-ordinator
DESCRIPTION:Understanding the organizing principles driving the assembly o
 f cellular organelles and of protein machines is a fundamental pursuit in 
 biology. The centriole is a microtubule-based organelle that is essential 
 for the formation of flagella\, cilia and centrosomes. The centriole has a
  signature nine-fold symmetrical arrangement of microtubules organized aro
 und a likewise symmetrical cartwheel. The cartwheel comprises a stack of r
 ing-containing elements that each accommodates 9 homodimers of SAS-6 prote
 ins. Although important advances have been made in recent years\, understa
 nding of centriole assembly remains incomplete\, partly due to the lack of
  cell-free assays to probe the dynamics of the process at nanometric scale
 . To fill this gap\, we have developed a high-speed atomic force microscop
 y (AFM) to reconstitute and monitor the assembly dynamics of SAS-6 protein
 s. Moreover\, to develop tools to dissect and modulate centriole assembly\
 , we have identified monobodies that recognize SAS-6 proteins with high af
 finity. Our analysis established that some of these monobodies prevent the
  assembly of ring-containing elements in vitro and of the centriole organe
 lle in cellulo. Together\, these approaches provide novel insights into th
 e mechanisms governing the assembly of the fundamental centriole organelle
 .
LOCATION:Webinar -  https://zoom.us/j/91953336333?pwd=ZnV2R1dNSW1FdVhmK1J6
 UmcvdnNOdz09
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