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SUMMARY:Crop Science Seminar: Revealing the “box” code: the spatial an
 d temporal regulation of plant-parasitic nematode pathogenicity - Clement 
 Pellegrin\, Marie Sklodowska-Curie European Fellow\, Crop Science Centre
DTSTART:20220406T093000Z
DTEND:20220406T103000Z
UID:TALK172298@talks.cam.ac.uk
CONTACT:Katherine Maltby
DESCRIPTION:Plant-parasitic nematodes are an important group of plant path
 ogens that threaten current and future food security. Among them\, the cys
 t nematodes parasitize some of the most important crop species. To cause d
 isease\, cyst nematodes inject effector proteins into the plant\, produced
  primarily in either the dorsal or sub-ventral glands. In order to identif
 y the genetic signatures and corresponding readers of gene expression in d
 ifferent gland cell and at different times of infection\, we used Pacbio D
 NA-seq and Illumina RNA-seq to reconstruct the genome and lifestage-specif
 ic transcriptome of Heterodera schachtii infecting the model plant Arabido
 psis thaliana. We then combined this transcriptome-wide analysis of tempor
 al expression with a gland cell specific RNAseq dataset to identify candid
 ate regulators of the gland cells. This led to the identification of a tra
 nscription factor regulating the subventral gland\, coined the SUGR. Funct
 ional analysis of the SUGR reveals it regulates a set of known and candida
 te subventral gland effectors as well as additional transcription factors.
  This suggests the SUGR is part of a cascade of regulation involved in pla
 nt penetration\, consistent with the reduction in plant penetration observ
 ed after RNAi-mediated silencing of the SUGR-encoding gene. Finally\, the 
 SUGR-encoding gene expression gets either activated or inhibited by plant-
 derived signals that remain to be identified\, shedding light on potential
  control strategies. \n\nDue to having to go online\, we are restricting t
 he talks to University of Cambridge and alumni to keep them as informal as
  possible.\n\nContact reception@plantsci.cam.ac.uk for a Zoom link prior t
 o a talk if you are not on our mailing list.
LOCATION:Online
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