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SUMMARY:Kendrew Lecture 2023: Cryo-electron tomography or the power of see
 ing the whole picture - Wolfgang Baumeister\, Max Planck Institute of Bioc
 hemistry\, Germany
DTSTART:20231030T110000Z
DTEND:20231030T120000Z
UID:TALK199738@talks.cam.ac.uk
CONTACT:Scientific Meetings Co-ordinator
DESCRIPTION:Traditionally\, structural biologists have approached cellular
  complexity in a reductionist manner by studying isolated and purified mol
 ecular components. This 'divide and conquer' approach has been highly succ
 essful\, as evidenced by the impressive number of entries in the PDB. \nHo
 wever\, awareness has grown in recent years that only rarely biological fu
 nctions can be attributed to individual macromolecules. Most cellular func
 tions arise from their acting in concert. Hence there is a need for method
 s developments enabling studies performed in situ\, i.e. in unperturbed ce
 llular environments. Sensu stricto the term 'structural biology in situ' s
 hould apply only to a scenario in which the cellular environment is preser
 ved in its entirety. Cryo electron tomography (Cryo ET) has unique potenti
 al to study the supramolecular architecture or 'molecular sociology' of ce
 lls. It combines the power of three-dimensional imaging with the best stru
 ctural preservation that is physically possible to achieve. \nWe have used
  cryo-electron tomography to study the 26S proteasome\, the main machinery
  for protein degradation\, in a number of cellular settings revealing its 
 precise location\, assembly and activity status as well as its interaction
 s with other molecular players of the cellular protein quality control mac
 hinery. Another pathway for the disposal of waste is autophagy. Cryo ET ha
 s allowed to visualize the biogenesis of autophagosomes in great detail. \
 nFor unlocking the full potential of cryo ET\, further advances in technol
 ogy and methodology are needed. \nBaumeister W.: Cryo-electron tomography:
  A long journey to the inner space of cells\, Cell\, 185(15):2649-2652 (20
 22)
LOCATION:In person in the Max Perutz Lecture Theatre (CB2 0QH) and via Zoo
 m\, link: https://mrc-lmb-cam-ac-uk.zoom.us/j/98196424925?pwd=a0V2Z1FOYU5U
 ZFgreHp1ckQ4Z2UyUT09
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