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SUMMARY:New Native Nanodiscs for Membrane-Protein Biophysics - Prof. Sandr
 o Keller\, University of Graz
DTSTART:20230623T130000Z
DTEND:20230623T140000Z
UID:TALK202564@talks.cam.ac.uk
CONTACT:Dr Georg Krainer
DESCRIPTION:Nanodiscs that harbour individual membrane proteins or membran
 e-protein complexes in a lipid-bilayer environment hold great promise for 
 biophysical investigations under well-controlled yet native-like condition
 s. Our laboratory focusses on new methods for the direct\, detergent-free 
 ex-traction of proteins from cellular membranes into native nanodiscs\, wh
 ich preserve both the over-all bilayer architecture and the local lipid co
 mposition of the original cellular membrane. Thus\, these native nanodiscs
  render membrane proteins amenable to in vitro biophysical investigations 
 without ever removing the proteins from a lipid-bilayer environment. Recen
 tly\, we have developed and used novel amphiphilic polymers with improved 
 properties for forming nanodiscs that are compatible with a broad range of
  ensemble and single-molecule biophysical techniques.[1–3] In this talk\
 , I will present selected examples including antibody–receptor and pepti
 de–lipid interactions studied by microfluidic diffusional sizing\, membr
 ane-protein complexes investigated by mass spec-trometry\, and cell-free t
 ranslation of membrane proteins with cotranslational insertion into lipid-
 bilayer nanodiscs.\n\n*References*\n\n[1] D. Glück\, A. Grethen\, M. Das\
 , O.P. Mmeka\, E. Pérez Patallo\, A. Meister\, R. Rajender\, S. Kins\, M.
  Räschle\, J. Victor\, C. Chu\, M. Etzkorn\, Z. Köck\, F. Bernhard\, J.O
 . Babalola\, C. Vargas\, S. Keller. Electroneutral polymer nanodiscs enabl
 e interference-free probing of membrane proteins in a lipid-bilayer enviro
 nment. Small 2022\, accepted\n\n[2] B. Danielczak\, M. Rasche\, J. Lenz\, 
 E. Pérez Patallo\, S. Weyrauch\, F. Mahler\, M.T. Agbadaola\, A. Meister\
 , J.O. Babalola\, C. Vargas\, C. Kolar\, S. Keller. A bioinspired glycopol
 ymer for capturing membrane proteins in native-like lipid-bilayer nanodisc
 s. Nanoscale 2022\, 5\, 1855\n\n[3] F. Mahler\, A. Meister\, C. Vargas\, G
 . Durand\, S. Keller. Self-assembly of protein-containing lipid-bilayer na
 nodiscs from small-molecule amphiphiles. Small 2021\, 17\, 2103603
LOCATION:Unilever Lecture Theatre\, Department of Chemistry
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