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SUMMARY:Wolfram Pönisch-Deciphering the role of noise in cell shape chang
 es during epithelial-to-mesenchymal transition\; Constance Le Gloanec-Prot
 ect and provide: the dual role of the cauline leaf. - Constance Le Gloanec
  and Wolfram Pönisch
DTSTART:20240603T133000Z
DTEND:20240603T143000Z
UID:TALK215920@talks.cam.ac.uk
CONTACT:Jia CHEN
DESCRIPTION: Wolfram Pönisch - Postdoc in Lab of Ewa Paluch\, PDN Univers
 ity of Cambridge \n\nTitle: Deciphering the role of noise in cell shape ch
 anges during epithelial-to-mesenchymal transition \n\nAbstract: The develo
 pment of an organism requires sequential state transitions towards more sp
 ecialised cell types. Many state transitions coincide with changes in cell
  shape\, with emerging evidence suggesting strong feedback between shape a
 nd state. An example of transitions where state and shape are tightly coup
 led is epithelial-to-mesenchymal transition (EMT) which plays a crucial ro
 le in development and pathogenesis. While the changes in gene expression d
 riving EMT have been extensively studied\, the cell shape dynamics during 
 EMT remain poorly understood. \n\nTo address this challenge\, we developed
  a morphometric pipeline employing spherical harmonics descriptors to repr
 esent 3D cell morphodynamics in a low-dimensional morphospace quantitative
 ly. Combining live-cell imaging with this pipeline\, we characterised the 
 cell shape trajectories associated with EMT. We inferred the underlying st
 ochastic morphodynamics by modelling shape dynamics as a Langevin process 
 and characterised the cell shape noise. Our findings reveal a peak in nois
 e coinciding with a transition from epithelial to mesenchymal attractor st
 ates. Molecular perturbation experiments and mathematical modelling sugges
 t that an increase in actin protrusivity and a decrease in membrane tensio
 n account for the cell shape noise during EMT. Together\, our study sugges
 ts that EMT-associated cell spreading can be described as a transition bet
 ween morphospace attractors. \n\n\nConstance Le Gloanec\, NUS\n\nTitle: Pr
 otect and provide: the dual role of the cauline leaf  \n  \nAbstract: Plan
 t organs have evolved diverse shapes for specialized functions despite eme
 rging as simple protrusions at the shoot apex. Cauline leaves serve both a
 s photosynthetic organs and protective structures for emerging floral buds
 . However\, their growth patterns remain elusive. Here\, we investigate th
 e developmental dynamics shaping cauline leaves underlying their functiona
 l diversification from other flat organs. We show that cauline leaves disp
 lay a strong delay in overall elongation as compared to juvenile leaves. U
 sing live-imaging\, we reveal that their functional divergence hinges on e
 arly modulation of the timing of cell differentiation and cellular growth 
 rates. In contrast to rosette leaves and sepals\, cell differentiation is 
 delayed in cauline leaves\, fostering extended proliferation\, prolonged m
 orphogenetic activity\, and growth redistribution within the organ. Notabl
 y\, cauline leaf growth is transiently suppressed during the early stages\
 , keeping the leaf small and unfolded during the initiation of the first f
 lowers. Our findings highlight the unique developmental timing of cauline 
 leaves\, underlying their shift from an early protective role to a later p
 hotosynthetic function. \n\nZoom link: Join Zoom Meeting https://cam-ac-uk
 .zoom.us/j/87939943699 Meeting ID: 879 3994 3699
LOCATION:Online
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