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SUMMARY:LMB Seminar - New Perspectives on CDKs and Cell Cycle Control - Si
 r Paul Nurse\, The Crick Institute
DTSTART:20260126T110000Z
DTEND:20260126T120000Z
UID:TALK240469@talks.cam.ac.uk
CONTACT:Scientific Meetings Co-ordinator
DESCRIPTION:The Cyclin Dependent Kinases (CDKs) are a family of master reg
 ulators of the eukaryotic cell cycle. Work from the lab has shown that the
  fission yeast cell cycle can be driven by a single CDK-cyclin complex\, a
 nd unexpectedly that the G1/S and G2/M CDKs have very similar substrate sp
 ecificities. This suggests that rising total CDK activity in the cell play
 s a major role in bringing about the correct temporal order of cell cycle 
 events.\n\nThe CDK-cyclin complex has a third CKS sub-unit (Suc1 in fissio
 n yeast) which we show enhances the phosphorylation of around 200 CDK phos
 phosites on 136 proteins\, many of which are required for the different ev
 ents of S-phase and mitosis. Ablation of Suc1 activity results in defects 
 in both S-phase and mitosis onset and progression. Suc1 also controls the 
 association of Wee1 and Cdc25 with CDK\, thus regulating CDK Y15 phosphory
 lation and activity at mitotic onset.\n\nSingle cell CDK sensor assays hav
 e shown that CDK is activated first in the nucleus and then later in the c
 ytoplasm. Nuclear CDK activation results in export of CDK into the cytopla
 sm activating the CDK located there. In vivo phase plots show that cyclin 
 control over CDK activation is stronger in the nucleus than the cytoplasm.
  We conclude that nuclear CDK acts as the ‘mitotic pacemaker’ determin
 ing when cells enter mitosis. Locating the CDK pacemaker in the nucleus pl
 aces it closer to DNA where it can be more readily controlled by the DNA c
 heckpoints and ploidy controls essential for genome stability. These exper
 iments emphasise the need to better understand spatial aspects of CDK regu
 lation in the cell in addition to temporal aspects.\n\n\n
LOCATION:In person in the Max Perutz Lecture Theatre (CB2 0QH) and via Zoo
 m link https://mrc-lmb-cam-ac-uk.zoom.us/j/97807820456?pwd=Zom7d6xnoBUHOEy
 OsTOQphtYGZTyGt.1
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