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SUMMARY:De novo assembly and evolutionary analyses of liver-expressed gene
 s in 16 mammal species - Marioni\, J (University of Chicago)
DTSTART:20100715T090000Z
DTEND:20100715T093000Z
UID:TALK25516@talks.cam.ac.uk
CONTACT:Mustapha Amrani
DESCRIPTION:Changes in gene regulation have been proposed as critical in t
 he evolution of phenotypic diversity of primates. However\, the lack of hi
 gh-quality reference genomes for most species and the limited number of in
 dependently derived transcripts for non-human primates has made it difficu
 lt to study gene regulation across multiple primates. To overcome these pr
 oblems\, we used massively-parallel sequencing to interrogate mRNA samples
  extracted from the livers of 16 species (12 primates including human\, an
 d 4 non-primate out groups\; 4 samples per species). Of the 12 primate spe
 cies\, 8 do not have currently available reference genome sequences (verve
 t\, galago\, slow loris\, and 5 lemur species)\, which means that we had t
 o assemble the transcriptomes de novo for these species. This study design
  results in nucleotide sequence\, quantitative expression\, and gene struc
 ture data from thousands of genes\, providing insight into gene regulation
  and sequence evolution across a broad spectrum of primate species. \n\nIn
  this talk\, I will describe the considerable statistical and computationa
 l challenges that arose when assembling transcriptomes de novo and how we 
 analyzed the data generated. I will also discuss sets of genes whose expre
 ssion level patterns are consistent with the action of natural selection a
 long individual or ancestral primate lineages\, and describe the relations
 hip between alternative splicing within species and complete exon gains an
 d losses between species. 
LOCATION:Seminar Room 1\, Newton Institute
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