BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:Transposed element RNAs detected by massively parallel sequencing 
 - Faulkner\, G (University of Edinburgh)
DTSTART:20100715T100000Z
DTEND:20100715T103000Z
UID:TALK25520@talks.cam.ac.uk
CONTACT:Mustapha Amrani
DESCRIPTION:Transposed elements (TEs) typically comprise 30-50% of the mam
 malian genome and\, until recently\, their repetitive composition has rest
 ricted our ability to study individual TEs. Here I will present our early 
 statistical modelling that indicated high throughput sequencing could be s
 uccessfully applied to detect transcription from specific TEs\, as well as
  a computational solution to the related multi-map tag phenomenon. These w
 orks formed the basis for a subsequent landmark publication proving that T
 Es were heavily transcribed in mammals. The associated transcripts\, dubbe
 d teRNAs\, supplied up to 30% of the capped RNA molecules in cancer\, germ
  and somatic cells. An analysis of 250 000 TE promoter regions revealed th
 at these frequently functioned as alternative promoters for nearby genes a
 nd/or expressed ncRNAs. Overall\, we discovered that the abundance of teRN
 As far exceeded prior estimates\, with a common theme of strong tissue spe
 cificity and association with nearby protein-coding genes\, demonstrating 
 that TEs were an essential part of the mammalian transcriptome and regulom
 e. 
LOCATION:Seminar Room 1\, Newton Institute
END:VEVENT
END:VCALENDAR