BEGIN:VCALENDAR VERSION:2.0 PRODID:-//Talks.cam//talks.cam.ac.uk// X-WR-CALNAME:Talks.cam BEGIN:VEVENT SUMMARY:Mitosis and Meiosis in live mammalian cells: From genome wide RNAi profiling to homologous chromosome segregation - Jan Ellenberg\, EMBL\, H eidelberg\, Germany DTSTART:20101105T141500Z DTEND:20101105T160000Z UID:TALK27449@talks.cam.ac.uk CONTACT:Scientific Meetings Co-ordinator DESCRIPTION:Despite our exponentially growing knowledge about the human ge nome\, we do not know all human genes required for some of the most basic functions of life such as somatic cell division. To start to fill this ga p we developed a high throughput phenotypic screening platform combining p otent gene silencing by RNA interference\, time lapse microscopy and compu tational image processing. We carried out a genome-wide phenotypic profii ling of each of the 21`000 human protein-coding genes by two day live imag ing of fluorescently labelled chromosomes. Quantitative phenotyping by co mputational image processing allowed us to identify hundreds of human gene s involved in cell division (www.mitocheck.org ) \, which we are now studying in mechanistic detail in follow up studies us ing automated advanced fluorescence microscopy methods.\n\nEven more than the molecular mechanisms of mitosis\, many questions about the segregation of homologous chromosomes during the formation of germ cells remain open. To study this process\, we have established complete kinetochore trackin g by high resolution 4D imaging of the first meiotic division in mouse ooc ytes. Quantitative analysis of this data reveals the highly error prone n ature of mammalian homologous chromosome biorientation that may provide a possible mechanistic explanation for the high incidence of chromosome aneu ploidies in mammalian eggs. LOCATION:Max Perutz Lecture Theatre\, Medical Research Council (MRC) (MRC Laboratory of Molecular Biol END:VEVENT END:VCALENDAR