BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:The role of small non-coding RNAs in mouse primordial germ cell sp
 ecification and epigenetic reprogramming - Delphine Cougot\, Gurdon Instit
 ute
DTSTART:20110311T130000Z
DTEND:20110311T133000Z
UID:TALK29372@talks.cam.ac.uk
CONTACT:15560
DESCRIPTION:Small non-coding RNAs have been shown to play critical roles i
 n mediating epigenetic modifications in regulating chromatin structures\, 
 which is likely to be important during the specification of primordial ger
 m cells (PGCs) in mice\, and during genome-wide epigenetic reprogramming i
 n early germ cells. Little is currently known about the role of small non-
 coding RNAs in early germ cells because of the limited numbers of availabl
 e cells for analysis. First\, the role of all non-coding RNAs in germ cell
 s specification and in the establishment of the specific and critical chro
 matin signature in E8.5 PGCs will be studied in a mouse model in which Arg
 onaute 2 (Ago2) is inactivated. Second\, we will investigate gonadal germ 
 cells at E10.5-E12.5 when they exhibit extensive epigenetic reprogramming\
 , including genome-wide DNA demethylation and chromatin remodelling. We wi
 ll use RNA-Seq to obtain comprehensive information on all non-coding RNAs\
 , which will help to identify particular types of small non-coding RNA tha
 t are likely involved in the regulation of chromatin structure. These data
  may also provide information on their putative target genes. To investiga
 te the function of small non-coding RNAs at these particular stages\, we w
 ill use mouse models in which Dicer or Ago2 are specifically deleted in ea
 rly PGC and we will analyse the effect of the inactivation of the RNAi pat
 hway on this global epigenetic reprogramming. Elucidating the function of 
 small non-coding RNA in these major epigenetic events is important for our
  understanding of the mechanisms involved in the generation of the totipot
 ent state.
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
END:VEVENT
END:VCALENDAR