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SUMMARY:The FRIGIDA complex-mediated FLC activation in winter annuals of A
 rabidopsis thaliana - Kyuha Choi
DTSTART:20110318T130000Z
DTEND:20110318T133000Z
UID:TALK30174@talks.cam.ac.uk
CONTACT:15560
DESCRIPTION:The winter annuals of Arabidopsis thaliana  require  vernaliza
 tion-mediated silencing of the strong flowering repressor FLOWERING LOCUS 
 C (FLC) for floral promotion competence. FRIGIDA (FRI) encoding a proline-
 rich protein with two coiled-coil domains increases transcript level of FL
 C\, but the molecular mechanism remains elusive. The natural mutations of 
 FRI cause an early flowering with reduced FLC expression in most of rapid 
 life cycle ecotypes including Col-0. Forward genetic approaches using the 
 winter annuals have identified additional early flowering frl1\, fes1\, su
 f4 and flx\, similar to fri as mutants of FLC-specific activators. We show
 ed that FRI acts as a scaffold protein interacting with FRL1\, FES1\, SUF4
 \, and FLX to form a transcription activator complex (FRI-C). Each compone
 nt of FRI-C has a specialized function. SUF4\, a C2H2 type zinc finger pro
 tein binds to a cis-element of the FLC promoter\, FLX and FES1 have transc
 riptional activation potential\, and FRL1 and FES1 stabilize the complex. 
 FRI-C recruits a general transcription factor\, a TAF14 homolog\, and chro
 matin modification factors\, the SWR1 complex and SET2 homolog. Complex fo
 rmation was confirmed by the immunoprecipitation of FRI-associated protein
 s followed by mass spectrometric analysis. Our results provide insight int
 o how a specific transcription activator recruits chromatin modifiers to r
 egulate a key flowering gene. 
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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