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SUMMARY:Visualizing vesicle and virus budding by electron microscopy - Joh
 n Briggs\, EMBL
DTSTART:20110601T103000Z
DTEND:20110601T121500Z
UID:TALK31583@talks.cam.ac.uk
CONTACT:Scientific Meetings Co-ordinator
DESCRIPTION:The budding of coated vesicles and membrane viruses can be stu
 died using new electron microscopy methods. I will illustrate how cryo-ele
 ctron tomography and sub-tomogram averaging can describe the structure and
  arrangement of protein coats in situ on vesicles and viruses\, and how co
 rrelative light and electron microscopy can identify intermediates in budd
 ing processes. I will present our results from applications of these metho
 ds to COPI\, clathrin and HIV Gag mediated budding. 
LOCATION:Max Perutz Lecture Theatre\, Medical Research Council (MRC) (MRC 
 Laboratory of Molecular Biol
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