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SUMMARY:mRNA-tRNA translocation intermediates by cryo-EM: a look at the fr
 ee-energy landscape from a single sample. - Joachim Frank\, Columbia Unive
 rsity
DTSTART:20110930T151500Z
DTEND:20110930T170000Z
UID:TALK32572@talks.cam.ac.uk
CONTACT:Scientific Meetings Co-ordinator
DESCRIPTION:After peptide bond formation (i.e.\, in the pre-translocationa
 l phase)\, the translating ribosome exists in multiple states that are in 
 equilibrium.  The states are characterized by different degrees of the rat
 chet-like intersubunit movement and different positions of the tRNAs\, fro
 m classical to fully hybrid.  Interconversion between these states is effe
 cted by Brownian motion\, and the productive "end state" is fixed by the i
 ntervention of EF-G.  Using cryo-EM of a single sample of the pre-transloc
 ational ribosome complex in the absence of EF-G\, we were able to reconstr
 uct the ribosome-tRNA configuration in several states.  The varying riboso
 me domain and tRNA positions are quantified by tensor analysis and compare
 d with those in the literature.  A one-dimensional free-energy profile can
  be deduced from the relative occupancies of the states. 
LOCATION:Max Perutz Lecture Theatre\, Medical Research Council (MRC) (MRC 
 Laboratory of Molecular Biol
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