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SUMMARY:Lessons from pathogens - identifying novel cell surface receptors 
 targeted by viruses - Professor Paul Lehner\, Cambridge Institute for Medi
 cal Research
DTSTART:20120215T123000Z
DTEND:20120215T133000Z
UID:TALK35124@talks.cam.ac.uk
CONTACT:Sue Griffin
DESCRIPTION:As the ultimate intracellular parasites\, viruses target the h
 ost cellular machinery to enable their replication and avoid elimination b
 y the immune system. We have taken functional genetic and proteomic approa
 ches to identify novel cellular receptors manipulated by viruses\, underst
 and why these receptors are affected and to elucidate the mechanisms virus
 es use to manipulate their cellular targets.  \n\nOur work on viral evasio
 n of the MHC class I antigen presentation pathway has driven an interest i
 n the role of ubiquitin in immunoreceptor regulation. For example\, the K3
  and K5 virally encoded ubiquitin E3 ligases recruit host E2 enzymes to ub
 iquitinate MHC class I molecules. These lys63-linked ubiquitin conjugates 
 initiate the downregulation and subsequent endolysosomal degradation of ce
 ll surface MHC I molecules. \n\nHowever\, a large number of cell surface r
 eceptors will be modulated by different intracellular pathogens. We theref
 ore developed an unbiased proteomics approach to identify those receptors 
 whose expression at the cell surface is altered upon viral infection. Usin
 g SILAC-based proteomics\, the identification and differential analysis of
  >600 plasma membrane proteins allows us to find those receptors downregul
 ated by viruses. \n\nThis approach has identified immune evasion as well a
 s metabolic receptors whose expression is dramatically altered following i
 nfection\, and will be discussed during the seminar.
LOCATION:Lecture Theatre\, Department of Pathology\, Tennis Court Road
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