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SUMMARY:Structural analysis of Arabidopsis cell wall arabinogalactan polys
 accharides - Dora Tryfona\, Dupree Lab\, Biochemistry
DTSTART:20120217T130000Z
DTEND:20120217T133000Z
UID:TALK35544@talks.cam.ac.uk
CONTACT:24938
DESCRIPTION:Plant type II arabinogalactan (AG) polysaccharides are attache
 d to core-proteins of arabinogalactan-proteins (AGPs) usually at hydroxypr
 oline residues. Due to their complex branching structures and tissue-\, ce
 ll- or perhaps protein-specificities\, type II AGs are overwhelmingly dive
 rse and heterogeneous. To gain insights into the synthesis and function of
  plant AGPs\, it would be useful to have mutants altered in their carbohyd
 rate moieties of cell wall constituents. Here\, enzymes able to release ol
 igosaccharides specifically from AGs\, high energy Matrix Assisted Laser D
 esorption Ionisation (MALDI)-Collision Induced Dissociation (CID) mass spe
 ctrometry (MS) and Polysaccharide Analysis by Carbohydrate gel Electrophor
 esis (PACE) were employed to study the carbohydrate component of Arabidops
 is leaf AGPs. The structures of AG-specific enzyme digestion products were
  analysed by PACE and MS. To our knowledge this is the first time a partia
 l structure for the carbohydrate component of Arabidopsis AGPs is proposed
 . In addition\, AG oligosaccharides from leaves of the Fuc-deficient mur1 
 mutant were identified and their structures were compared with those isola
 ted from wild-type plants. We show that in mur1 mutant\, AGPs are lacking 
 Fuc modifications\, demonstrating that Arabidopsis mutants in AGP structur
 e can be identified and characterised. 
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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