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SUMMARY:MHC haplotyping in rhesus and cynomolgus macaques - Dr. Gaby Doxia
 dis\, BPRC Rijswijk\, The Netherlands
DTSTART:20120509T113000Z
DTEND:20120509T123000Z
UID:TALK37419@talks.cam.ac.uk
CONTACT:Sue Griffin
DESCRIPTION:The Biomedical Primate Research Centre in Rijswijk\, The Nethe
 rlands\, houses breeding colonies of rhesus as well as cynomolgus macaques
  for biomedical and ethological research\, respectively.\n \nThe gene prod
 ucts of the Major Histocompatibility complex (MHC) play an important role 
 in immune reactions\, and the genes encoding these molecules show a high d
 egree of variability and diversity in humans as well as non-human primates
 . The classical Mhc genes of rhesus (Macaca mulatta) and cynomolgus (Macac
 a fascicularis) macaques\, Mamu/Mafa-A\, -B\, and -DRB show several levels
  of complexity\, namely allelic variation (polymorphism)\, copy number var
 iation\, the combination of different genes per haplotype (diversity)\, an
 d transcription level variation as well. \n\nIn the past\, more than five 
 generations of monkeys of the rhesus macaque breeding colony of Indian ori
 gin have been serologically typed\, and MHC haplotypes could be defined ba
 sed on segregation. Nowadays\, serology has been replaced by molecular typ
 ing methods as genomic exon 2 sequencing (Mamu-DQA1\, -DQB1\, -DPB1\, -DRB
 ) and full-length cDNA analysis (Mamu-A and -B). \n\nRecently\, microsatel
 lite typing has been added to the methods that allow haplotyping in an eff
 icient\, robust\, time saving and inexpensive manner. In such a way\, 15 d
 ifferent Mamu-A\, 17 –B\, and 21 –DRB (haplo)types could be defined wi
 thin a panel of more than 1000 Indian rhesus macaques which make up a tota
 l of 135 different MhcMamu haplotypes. The same typing methods led to the 
 definition of 32 different Mhc haplotypes within the cynomolgus macaque br
 eeding colony of < 200 monkeys.\n \nOur data show that microsatellite typi
 ng methods are useful to define Mhc haplotypes for both macaque species. F
 urthermore\, the results demonstrate that the MHC of the cynomolgus macaqu
 e appears to consist of unique A/B/DR (haplo)types whereas the MHC of the 
 Indian rhesus macaque seems to be built up by a combination of several A\,
  B\, and DR segments.\n\n
LOCATION:Lecture Theatre\, Department of Pathology\, Tennis Court Road
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