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SUMMARY:Membrane biology in plant-microbe interactions - Silke Robatek\, J
 IC
DTSTART:20130314T130000Z
DTEND:20130314T140000Z
UID:TALK39046@talks.cam.ac.uk
CONTACT:Jill Harrison
DESCRIPTION:All plant organs are vulnerable to colonisation and molecular 
 manipulation by microbes. In our attempts to understand the full nature of
  the interactions that occur between a potential pathogen and its host\, w
 e are focussing on membrane biology in plant-microbe interactions. Immune 
 receptors constitute recognition sites to detect invading pathogens and to
  trigger defenses\, and the activity of these receptors depends upon the d
 ynamic membrane trafficking network. The plasma membrane receptor FLAGELLI
 N SENSING 2 (FLS2) confers plant immunity through perception of bacterial 
 flagellin (flg22). Following elicitation\, FLS2 is internalized into vesic
 les. To resolve FLS2 trafficking\, we exploited quantitative high-throughp
 ut confocal imaging for co-localisation studies and chemical interference.
  FLS2 localises to bona-fide endosomes via two distinct endocytic traffick
 ing routes depending on its activation status. I will present our high-thr
 oughput confocal imaging pipeline to study endosomal pathways in plants an
 d will discuss results from our current research\, which addresses the mol
 ecular components regulating FLS2 endocytosis\, and the intersection betwe
 en FLS2 endocytosis and flg22 signaling. To further study receptor-mediate
 d endocytosis in plant immunity we focus on the receptors EFR and PEPR1 fo
 r bacterial EF-Tu (elf18) and the endogenous elicitor pep1\, respectively\
 , as well as the co-regulator BAK1/SERK3. I will describe recent results d
 emonstrating that ligand-induced internalization of FLS2 represents a cons
 erved endocytic trafficking pathway. Altogether\, high-throughput confocal
  imaging combined with functional studies allows us to tackle the dynamic 
 cellular changes involved in the interaction between plants and microbes.
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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