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SUMMARY:The yeast Pif1 family helicase RRM3 promotes DNA unwinding during 
 replisome swivelling - Baxter\, J (University of Sussex)
DTSTART:20120905T142000Z
DTEND:20120905T144000Z
UID:TALK39546@talks.cam.ac.uk
CONTACT:Mustapha Amrani
DESCRIPTION:During termination of DNA replication\, replisomes overcome th
 e topological tension that occurs as forks converge by coupling final unwi
 nding with fork swivelling. Here I show that cells lacking the DNA helicas
 e RRM3 accumulate terminating late replication intermediates (LRI) in plas
 mids both with and without characterised pause sites. Rrm3 deletion does n
 ot alter the level of swivelling that occurs during termination but its de
 pletion extends the lifetime of LRI while its over-expression leads to the
  rapid unwinding of the LRI. Therefore RRM3 promotes DNA unwinding when th
 e replisome swivels during termination. Potentially\, this activity is als
 o generally utilised during DNA replication to bypass topological blocks.\
 n
LOCATION:Seminar Room 1\, Newton Institute
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