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SUMMARY:Perspectives on Next Generation DNA Sequencing. - Dr Harold Swerdl
 ow from Wellcome Trust Sanger Institute 
DTSTART:20130110T143000Z
DTEND:20130110T153000Z
UID:TALK41983@talks.cam.ac.uk
CONTACT:Caroline Newnham
DESCRIPTION:The R+D department at the Wellcome Trust Sanger Institute (San
 ger Centre) has been exploring new methods to enhance the DNA sequencing p
 roduction pipeline\, largely based upon Illumina technology. Work is under
 way on numerous projects including:\n•	Improving no-PCR library-preparat
 ion and other methods to eliminate bias\n•	Reducing contaminating human 
 DNA impacting the efficient sequencing of pathogens from clinical isolates
 \n•	A method for mapping transposon-insertion sites useful to identify e
 ssential genes\n•	A new protocol for long-insert (mate-pair) sequencing\
 n\nWe are simultaneously exploring newer next-generation technologies that
  have the potential to replace or complement the existing methods. Our exp
 erience with the PacBio instrument for de novo assembly and epigenetics wi
 ll be shown along with a novel method for direct sequencing of small genom
 es without library preparation. For fast sequencing of amplicons and bacte
 rial pathogens\, Life Technology’s Ion Torrent PGM has been tested and w
 ill be compared to Illumina’s benchtop competitor\, the MiSeq. The outlo
 ok for both Ion Torrent’s new instrument\, the Proton\, and Oxford Nanop
 ore’s technology will also be discussed.\n\n
LOCATION:Part II Room\, Department of Genetics
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