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SUMMARY:How to spot a Daisy - Gregory Mellers
DTSTART:20131108T130000Z
DTEND:20131108T132500Z
UID:TALK48217@talks.cam.ac.uk
CONTACT:Megan Cooper
DESCRIPTION:Our study aims to investigate how petal spots develop in the m
 odel species Gorteria diffusa. Previous work has found there to be multipl
 e “morphotypes” of the species which represent a series of natural mut
 ations fixed within discrete populations. These allow for comparative anal
 yses to be undertaken with the intention of elucidating the molecular regu
 lation of spot generation. Some evidence suggests a role for MYB genes in 
 the spot formation process hence comparative expression analyses (qPCR) an
 d heterologous expression studies (Nicotiana tabacum\, Senecio vulgaris) a
 re being undertaken. Furthermore\, multiple forms of microscopy (Light\, T
 EM) are being employed to further characterise the morphology of the spot 
 in the hopes of identifying other candidate regulatory genes. Restriction 
 site Associated DNA (RAD) sequencing is also being used to acquire high SN
 P coverage in an attempt to understand the relationship between the aforem
 entioned morphotypes. It is hoped that through these diverse techniques a 
 hypothetical model for spot formation may be found and subsequently pertur
 bed for validation.
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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