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SUMMARY:Directing Crossover During Meiosis Using TALENs - Patrick Diaz (He
 nderson Lab)
DTSTART:20140425T120000Z
DTEND:20140425T122500Z
UID:TALK51397@talks.cam.ac.uk
CONTACT:Megan Cooper
DESCRIPTION:Crossover is a highly conserved process in eukaryotes. For exa
 mple\, all species use the SPO11 endonuclease to generate DNA double stran
 d breaks (DSBs)\, which are repaired as COs. TAL-effector nucleases (TALEN
 s) consist of a TAL-effector DNA binding domain fused to a nuclease domain
 . The DNA binding domain contains a stretch of approximately 30 tandem rep
 eats. Each repeat contains two consecutive amino acid positions which are 
 hyper variable – termed the repeat variable diresidue (RVD). Every repea
 t binds a single base of DNA and the base specificity of the repeat is det
 ermined by the identity of the RVD (NI:A\, NG:T\, NN:G\, HD:C). This ciphe
 r allows for the creation of TALENs targeted to practically any DNA sequen
 ce.\n\nIn this project we aim to develop a system where artificial DSBs ca
 n be directed to specific sequences during meiosis to manipulate CO patter
 ns. In order to create sequence-directed DSBs we will make use of TALENS. 
 This will be used as a tool to investigate control of CO patterns and will
  also serve as proof-of-principle for crop breeding technologies.
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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