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SUMMARY:Design of a synthetic transcriptional AND gate in the chloroplast 
 of Marchantia polymorpha - Christian Boehm (Haseloff lab)
DTSTART:20140509T123000Z
DTEND:20140509T125500Z
UID:TALK51406@talks.cam.ac.uk
CONTACT:Megan Cooper
DESCRIPTION:The chloroplast is a highly attractive chassis for synthetic b
 iology and metabolic engineering applications. To implement spatiotemporal
  control of gene expression in this organelle\, we develop a synthetic tra
 nscriptional AND gate based on split T7 RNA polymerase (T7RNAP) in the sim
 ple model plant Marchantia polymorpha. A chloroplast-encoded fluorescent r
 eporter under control of a T7 promoter will only be expressed if both the 
 N- and C-terminal fragments of split T7RNAP\, induced by two different inp
 ut signals in the nucleus\, are targeted to the chloroplast at the same ti
 me. As both the two independent input signals and the output of the AND ga
 te may be chosen arbitrarily\, this system may be adopted for various futu
 re applications in scope of developmental studies and metabolic engineerin
 g where spatiotemporal control of plastid gene expression is desired. Here
 \, we report our progress in the design and characterization of the split 
 T7RNAP system in Escherichia coli\, and the identification of a functional
  chloroplast-targeting peptide in Marchantia polymorpha. 
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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