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SUMMARY:Analysis of the C. reinhardtii THI4 riboswitch expression platform
   - Devinn Lambert
DTSTART:20140606T120000Z
DTEND:20140606T122500Z
UID:TALK51409@talks.cam.ac.uk
CONTACT:Megan Cooper
DESCRIPTION:\nThe binding of thiamine pryrophosphate (TPP) to the aptamer 
 of the THI4 riboswitch redirects expression platform alternative splicing 
 to include an upstream open reading frame (uORF) in the transcript\; the u
 ORF precedes the THI4 codon sequence thereby interfering withTHI4 translat
 ion.  There has been thorough study of the nucleotides and secondary struc
 tures of the THI4 aptamer\, but less attention dedicated to residues in th
 e expression platform.  In particular\, are the expression platform nucleo
 tides active or passive in riboswitch regulated alternative splicing?  Do 
 nucleotypic characteristics of the expression platform\, including size an
 d GC content\, influence regulation?  Can the uORF be modified?  Through t
 he assembly of six different THI4 constructs and phenotypic analysis in C.
  reinhardtii this presentation addresses the abovementioned questions as w
 ell as highlights the implications for using the THI4 riboswitch as a synt
 hetic biology tool. 
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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