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SUMMARY:Roles of cytoskeleton in hippocampal synaptic plasticity - Dr. Yas
 unori Hayashi\, Brain Science Institute\, RIKEN\, Japan
DTSTART:20160202T160000Z
DTEND:20160202T170000Z
UID:TALK57523@talks.cam.ac.uk
CONTACT:Ingo Greger
DESCRIPTION:Synaptic plasticity occurs as a result of biochemical reaction
 s and protein interactions that take place within small volume of less tha
 n 1 fl.  Application of traditional biochemical approaches is simply impra
 ctical to elucidate the process of synaptic plasticity.  We therefore empl
 oyed optical methods including FRET\, FLIM\, photoactivatable proteins and
  caged compounds to elucidate the mechanism of synaptic plasticity\, with 
 an emphasis on structural modification of dendritic spine seen during long
 -term potentiation (LTP) of hippocampal CA1 pyramidal neurons.  After the 
 induction of LTP\, the remodeling of actin cytoskeleton and its polymeriza
 tion is the first thing to happen.  Active cofilin is massively transporte
 d to the spine and severs the F-actin\, which likely generates new free en
 ds of actin from where new filament starts growing. Thereafter\, these two
  proteins forms a stable complex at the base of spine head thereby forming
  a stable F-actin and regulating spine expansion. In contrast\, the postsy
 naptic density (PSD) was independently remodeled\, as PSD scaffolding prot
 eins did not change their amount and localization until late protein synth
 esis-dependent third phase. Our findings show how and when spine substruct
 ures are remodeled during LTP and explain why synaptic plasticity rules ch
 ange over time.  I will also cover a recent work on quantitative analysis 
 of AMPAR phosphorylation in relation to synaptic plasticity.
LOCATION:Klug Seminar Room\, Level 2 2A180\, MRC LMB
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