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SUMMARY:Transcriptional regulation and downstream program of Hox and Cdx g
 enes during axial development in the mouse embryo. - Dr Jacqueline Descham
 ps\, Hubrecht Institute\, The Netherlands
DTSTART:20151029T140000Z
DTEND:20151029T150000Z
UID:TALK60347@talks.cam.ac.uk
CONTACT:Caroline Newnham
DESCRIPTION:I will present our recent data on the characterization of the 
 regulatory landscape orchestrating the transcriptional initiation and earl
 y collinear expression of the clustered Hox genes\, emblematic transcripti
 on factor-encoding genes that pattern the embryonic body. Upon mapping chr
 omatin marks and tridimensional interactions in the megabases-long genomic
  region flanking the Hox clusters on their “early” side\, we uncovered
  a number of regulatory elements potentially accounting for the priming an
 d the early transcription of the Hox genes in the posterior primitive stre
 ak. We validated the importance of some of these enhancers of the HoxA clu
 ster in transgenic reporter assays in vivo and after CRISPR Cas9-mediated 
 deletion in Epiblast stem cells. The data suggest a scenario underlying th
 e triggering of initial transcription and the sequential turning on of the
  clustered Hox genes in the early mouse embryo.\n\nIn the second part of t
 he seminar I will address the issue of the downstream program of Hox and C
 dx genes. These genes derived from the same Proto Hox ancestors and share 
 a function in the generation and antero-posterior patterning of nascent em
 bryonic tissues. Our previous data suggested that they are involved in mai
 ntaining the niche of progenitors for axial tissues. I will present our re
 cent results on the identification of Cdx downstream targets likely mediat
 ing this activity.  \n
LOCATION:Biffen Lecture Theatre\, Department of Genetics\, Downing Site
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