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SUMMARY:Assessment of broad-spectrum in vitro screening for toxicity profi
 ling: Bioactivity space of target focussed and phenotypic assays - Samar M
 ahmoud
DTSTART:20160422T110000Z
DTEND:20160422T112000Z
UID:TALK62969@talks.cam.ac.uk
CONTACT:Alex Thom
DESCRIPTION:Traditional methods for hazard identification rely heavily on 
 testing compounds on animals through\nexposure to acute and chronic repeat
 ed doses. However\, these methods are economically inefficient\nand provid
 e minimal information on the mechanistic background of the tested chemical
 s. Broad-scale\nin vitro screening was introduced as a potential alternati
 ve to animal testing for toxicity profiling and\nanimal testing prioritisa
 tion. Hence\, the ToxCast program was launched by the United States\nEnvir
 onmental Protection Agency (EPA) in 2007 to adopt large scale in vitro tes
 ting through cell-free\nand cell-based approaches. Yet\, the efficiency of
  in vitro screening for identifying hazards in\nchemicals is questionable.
  Here\, the similarity and diversity of the dataset were investigated\, gi
 ving\nconsiderable attention to compare target focussed assays against phe
 notypic screening. We found that\ntarget-based assays clustered together i
 n the bioactivity space compared to the dispersed phenotypic screens.\nInt
 erestingly\, nuclear receptor assays\, that had significant associations w
 ith toxicity in human\,\ncorrelated broadly with phenotypic assay measurem
 ents. In contrast\, nuclear receptor assays\, with low\ncorrelations with 
 bioactivities from phenotypic assays\, had low associations with human tox
 icity. Based on our observations\, we concluded that assay set selection i
 s recommended to profile toxicity of a specific biological type in compari
 son to the utility of broad-scale in vitro measurements at a time.
LOCATION:Unilever Lecture Theatre\, Department of Chemistry
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