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SUMMARY:Phage Sf6 ejection mechanisms - Professor Kristin N. Parent\, Mich
 igan State University\, East Lansing\, United States
DTSTART:20170117T153000Z
DTEND:20170117T160000Z
UID:TALK69678@talks.cam.ac.uk
CONTACT:Ilana Spilka
DESCRIPTION:Outer membrane proteins A and C mediate Sf6 infection by drama
 tically increasing the rate and efficiency of genome ejection. I will desc
 ribe a combination of in vivo studies with omp null mutants of Shigella fl
 exneri\, including classic phage assays and time-lapse fluorescence micros
 copy to monitor genome ejection at the single virion level\, and cryo-elec
 tron tomography of phage “infecting” outer membrane vesicles. In addit
 ion\, mutagenesis studies have demonstrated key regions in OmpA that media
 te infection. We conclude that Sf6 phage entry utilizes either outer membr
 ane proteins A or C\, with outer membrane protein A being the preferred re
 ceptor.
LOCATION:Online
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