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SUMMARY:Light sheet fluorescence microscopy for fast volumetric imaging of
  bio-samples - Pablo Loza-Alvarez. Staff Scientist SLN Chief at ICFO - The
  Institute of Photonic Sciences. Castelldefels (Barcelona)\, Spain 
DTSTART:20170526T150000Z
DTEND:20170526T160000Z
UID:TALK69888@talks.cam.ac.uk
CONTACT:Dr. Hernandez-Ainsa
DESCRIPTION:Light sheet fluorescence microscopy (LSFM) is a convenient too
 l for high resolution\, 3D\, imaging as it efficiently collects the genera
 ted fluorescence while at the same time minimizes photobleaching. Being ba
 sed on an intrinsic plane illumination\, it allows for a fast 2D imaging. 
 Therefore\, LSFM has been put forward as an interesting candidate for fast
  volumetric (3D) imaging.\n\nI will present a LSFM microscope which is com
 bined with the use of wavefront coding (WFC) techniques for achieving fast
  3D imaging. WFC is used to extend the depth of field (DOF) of the collect
 ing objective in a LSFM. This result in a system in which the light sheet 
 can be axially scanned through the biological sample (which remains static
 ). This provides LSFM with intrinsic volumetric imaging capabilities for v
 isualising the fast dynamics in 3D of living samples such as C. elgans wor
 ms\, Drosophila melanogaster fly embryos and Danio rerio zebrafish.
LOCATION:Small Lecture Theatre\, Cavendish Laboratory
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