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SUMMARY:Cellular BASICs and Particle Biophysics for Systems Biology - Prof
 .  Dr. Luke P. Lee\, Department of Biosystems Science & Engineering\, ETH 
 Zurich\, Biomolecular Nanotechnology Center\, Dept. of Bioengineering\, UC
  Berkeley
DTSTART:20070618T133000Z
DTEND:20070618T143000Z
UID:TALK7521@talks.cam.ac.uk
CONTACT:spl37
DESCRIPTION:Cellular Biologic Application Specific Integrated Circuits (BA
 SICs) [1-8] are developed for quantitative systems biology\, molecular med
 icine\, and drug discovery.  Soft-state BASICs are created by connecting e
 xisting and novel nano- or microfluidic circuits for biological analysis i
 n new ways.  We are creating a library of these "building blocks" to devel
 op multifunctional biological microprocessor on-a-chip.  In order to build
  a solid foundation of future quantitative and systematic drug discovery\,
  we have established critical modules of BASIC such as Integrated Multiple
  Patch-clamp Array Chip Technology (IMPACT) [1\, 2]\, integrated dynamic c
 ell culture and multiplexed bioreactors [3]\, integrated cell lysing [4]\,
  cell separation [5]\, single cell electroporation [6]\, dynamic single ce
 ll analysis [7\, 8]\, cell-cell communication biochips [9]\, and artificia
 l liver on-a-chip.\n\nParticle biophysics will be the foundation of the sy
 stematic studies of living cells.  Gold quantum nanoplasmonic particles ar
 e ideal probes for nanoscale spectroscopic molecular imaging\, phototherma
 l therapeutic\, and gene regulation applications [10-13].  Nanophotonic cr
 escents have structures with a sub-10 nm sharp edge\, which can enhance lo
 cal electromagnetic field at the edge area. The formation of unconventiona
 l nanophotonic crescent structure is accomplished by the interfacing both 
 bottom-up and top-down methods\, which allows an effective batch nanofabri
 cation and precise controls of surface enhanced Raman scattering (SERS) ho
 t spot coupling nanogap.  The nanocrescent probes can be used for label-fr
 ee molecular detection and understanding electron transfers of biomolecule
 s.  In this talk I will also describe our recent work on the design\, synt
 hesis and characterizations of molecular optical switches and PRET (Plasmo
 nic Resonance Energy Transfer) nanospectroscopic imaging which might impac
 t on the areas of nanobiology\, control of gene regulation & protein expre
 ssion\, bioimaging\, systems biology\, and drug discovery.\n\n\n[1]	Cristi
 an Ionescu-Zanetti\, et al.\, “Mammalian Electrophysiology on a Microflu
 idic Platform\,” PNAS 102(26)\, 9112-9117 (2005).\n[2]	Adrian Y. Lau\, e
 t al.\, "Open-access microfluidic patch-clamp array with raised lateral ce
 ll trapping sites\," Lab Chip\, 6\, 1510 (2006) \n[3]	Paul Hung\, et al. 
 “A Continuous Perfusion Microfluidic Cell Culture Array for High Through
 put Cell-based Assays\,” Biotechnology & Bioengineering\, 89\, 1-8 (2005
 ).\n[4]	Dino Di Carlo\, et al.\, “On-Chip Cell Lysis by Local Hydroxide 
 Generation\,” Lab on a Chip 5 (1) (2005).\n[5]	Wesley C. Chang\, et al. 
 “Biomimetic Technique for Adhesion-based Collection and Separation of Ce
 lls in a Microfluidic Channel\,” Lab on a Chip\, 5 (1)\, 64-73 (2005).\n
 [6]	Michelle Khine\, et al.\, “A Single Cell Electroporation Chip\,” L
 ab on a Chip\, 5 (1)\, 38-43 (2005).\n[7]	Dino Di Carlo et al. "Dynamic Si
 ngle-Cell Analysis for Quantitative Biology\," Anal Chem\, 78\, 7918-7925 
 (2006).\n[8]	Dino Di Carlo\, Liz Y. Wu\, Luke P. Lee\, "Dynamic single cel
 l culture array\," Lab Chip\, 6\, 1445-1449 (2006).\n[9]	Philip J. Lee\, e
 t al. “Microfluidic Application Integrated Device for Monitoring Direct 
 Cell-Cell Communication via Gap Junctions Between Individual Cell Pairs\,
 ” Appl. Phys. Lett. 86\, 223902 (2005).\n[10]	Yu Lu\, et al. “Nanophot
 onic Crescent Moon Structures with Sharp Edge for Ultrasensitive Biomolecu
 lar Detections by Local Electromagnetic Field Enhancement Effect\,” Nano
  Letters\, 5(1)\, 119-124 (2005).\n[11]	Gang L. Liu\, et al. "Magnetic Nan
 ocrescents as Controllable Surface Enhanced Raman Scattering Nanoprobes fo
 r Biomolecular Imaging\," Advanced Materials\, 17\, 2683-2688 (2005).\n[12
 ]	Gang Liu et al. "Optofluidic Control via Photothermal Nanoparticles\," N
 ature Materials 5(1)\, 27-32 (2006).\n[13]	Gang Liu et al. "A nanoplasmoni
 c molecular ruler for measuring nuclease activity and DNA footprinting\," 
 Nature Nanotechnology\, 1\, 47-52 (2006).\n
LOCATION: IRC in Superconductivity Seminar room
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