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SUMMARY:Kendrew Lecture- Illuminating biology at the nanoscale and systems
  scale by imaging  - Xiaowei Zhuang 
DTSTART:20180115T160000Z
DTEND:20180115T170000Z
UID:TALK76081@talks.cam.ac.uk
CONTACT:Scientific Meetings Co-ordinator
DESCRIPTION:Dissecting the inner workings of a cell requires imaging metho
 ds with molecular specificity\, molecular-scale resolution\, and dynamic i
 maging capability such that molecular interactions inside the cell can be 
 directly visualized. However\, the diffraction-limited resolution of light
  microscopy is substantially larger than molecular length scales in cells\
 , making many sub-cellular structures difficult to resolve. Another major 
 challenge in imaging is the relatively low throughput in terms of the numb
 er of molecular species that can be simultaneously imaged\, and genomic-sc
 ale throughput is desired for investigating many systems level questions. 
 In this talk\, I will describe two imaging methods that overcome these cha
 llenges and their biological applications. I will first describe stochasti
 c optical reconstruction microscopy (STORM)\, a super-resolution imaging m
 ethod that overcomes the diffraction limit. This approach has allowed mult
 icolor and three-dimensional imaging of living cells with nanometer-scale 
 resolution. I will present both technological advances and biological appl
 ications of STORM\, with focus on some recent discoveries of novel sub-cel
 lular structures enabled by STORM. I will also describe our recently devel
 oped single-cell transcriptome imaging method\, multiplexed error-robust F
 ISH (MERFISH)\, which allows thousands of RNA species to be quantified in 
 a spatially resolved manner in individual cells. This approach enables sin
 gle-cell transcriptomic analysis in the native context of tissues\, facili
 tating the delineation of gene regulatory networks\, the mapping of RNA di
 stributions inside cells\, and the mapping of distinct cell types in compl
 ex tissues. In addition\, multiplexed FISH allowed us to trace the folding
  path of individual chromosomes\, which has provided novel insights into t
 he 3D organization of the genome.
LOCATION:Max Perutz Lecture Theatre\, Medical Research Council (MRC) (MRC 
 Laboratory of Molecular Biol
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